Bio One Logo

Bio-One

Industry
Miscellaneous
Number of Open Locations
130
Start-Up Costs
$116,295 – $169,295
Part-Time Ownership?
No
Item 19 Available?
Yes
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Overview

Bio-One is the industry leader in biohazard and decontamination cleanup services, providing essential support to communities in their times of need. Founded in 2006 and franchising since 2010, Bio-One has grown to over 130 locations across 45 states. Franchisees offer specialized cleaning services, including crime scene cleanup, hoarding remediation, mold removal, and biohazard decontamination. Operating with discretion and compassion, Bio-One follows the mission of "Help First, Business Second," ensuring that communities receive much-needed services in difficult circumstances.

As a franchise owner, revenue is generated through a variety of high-value, insurance-based jobs. The business model is designed for low overhead and scalability, with no need for a brick-and-mortar location. Franchisees start with a small team of trained technicians and leverage Bio-One’s strong support system, industry partnerships, and marketing resources to grow their customer base. With demand for decontamination services remaining steady regardless of economic conditions, Bio-One offers a recession-resilient opportunity with multiple revenue streams.

This franchise is ideal for community-driven individuals who want to make a meaningful impact. No prior industry experience is required, but successful owners are strong leaders with business, sales, or operations management experience. Daily responsibilities include marketing, client outreach, managing service technicians, coordinating with insurance companies, and overseeing decontamination jobs.

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Quick Facts

Franchising Since
2010
Number of Open Locations
130
HQ Location
Littleton, CO
Home Based
Yes
Financing Available
Yes

Financial Requirements

Liquidity Requirement
$60,000
Net Worth Requirement
$100,000
Franchise Fee
$60,000
Veteran Discount Available?
Yes
Start-Up Costs
$116,295 – $169,295

Disclosed Financials

Does this franchise disclose financial performance?
Yes
Financial Summary

At this time, this franchise does not have any financial performance information that can be publicly disclosed. However, we understand the importance of financial insights when evaluating a franchise opportunity. Please reach out to us, and we’ll be happy to provide additional details to help you make an informed decision.

Training & Support

Initial Training Provided
Yes
Ongoing Support
Yes
Additional Details

New franchisees undergo hands-on training at Bio-One’s headquarters, where they learn pricing, marketing, estimating jobs, and field operations. This includes OSHA safety training and simulated biohazard job scenarios to ensure proper decontamination techniques.

Franchisees receive continuous support from Bio-One’s corporate team, including sales coaching, regional meetings, private franchisee groups, and access to a billing specialist who works with insurance adjusters. Marketing resources include SEO, PPC advertising, and social media support to drive brand awareness and customer acquisition.

Differentiators

  • First-to-Market Franchise Leader – Bio-One was the first franchise in the biohazard and decontamination industry, setting the standard for service excellence. 
  • Recession-Resilient & Year-Round Demand – Services are needed regardless of economic conditions, ensuring steady business opportunities. 
  • Low Overhead & Home-Based Model – No expensive real estate or large staff is required, keeping startup and operational costs manageable. 
  • Multiple Revenue Streams – Bio-One offers a wide range of services, including crime scene cleanup, hoarding remediation, and mold removal, creating diverse income opportunities. 
  • Comprehensive Training & Corporate Support – Franchisees receive extensive hands-on training, ongoing coaching, and a dedicated corporate team to assist with marketing, operations, and insurance billing.

Ideal Owner

Allows Part-Time Owners?
No
Ideal Candidate Summary

The ideal Bio-One franchisee is passionate about helping people and providing essential services to their community. No industry experience is necessary, but business, sales, or operations management experience is beneficial. Successful owners are strong communicators, relationship builders, and problem solvers who can lead a team and handle client interactions with professionalism and empathy.

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